Sodium dodecyl sulfate other means of identification product no 1894, 4095, l050 recommended use and restriction on use recommended use. Sodium lauryl sulphate material safety data sheet cas. It is a key component of many reagents used to purify nucleic acids because of its abilities to quickly disrupt the tissue architecture and to inhibit both rnase and. View or download the sodium dodecyl sulfate msds material safety data sheet or sds for 817034 from merck. Mechanism of interaction of sodium dodecyl sulfate with mouse. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910.
By incubation with sodium dodecyl sulfate and simultaneous boiling 95 degrees celsius. Sodium dodecyl sulfate electrophoresis revision date 20may2014 disclaimer the information provided on this safety data sheet is correct to the best of our knowledge, information and belief at the date of its publication. Plastic microtiter plate coated with anti human adiponectin monoclonal antibody 5. Us state regulations california proposition 65 this product does not contain any substances known to the state of california to cause cancer, developmental andor reproductive harm. Sodium dodecyl sulfate safety overview national diagnostics. Comparison between procedures using sodium dodecyl sulfate for shotgun proteomic analyses of complex samples michael s. Significant symptoms of exposure can persist for more than two years and can be. Anhydrous sodium sulphate ar sodium chloride ar procedure. Sodium dodecyl sulfate sds, white powder, electrophoresis, fisher bioreagents poly pail. Safety data sheet ghs product identifier other means of identification section 1. Sodium dodecyl sulfate cas 1512 ph eur find msds or sds, a coa, data sheets and more information. Sodium dodecyl sulfate sds, 10% solution not available. Sodium dodecyl sulfate l3771 product information sheet. Safety summary see msds for complete information before using product.
Dodecyl sulfate is incompatible with strong oxidizers. Suppression of sodium dodecyl sulfate polyacrylamide gel electrophoresis sample preparation artifacts for analysis of igg4 halfantibody. To remove sds from protein samples, it is recommended to use product no. Dodecyl benzene sulphonic acid sodium salt cas no 25155300 material safety data sheet sds msds. Dodecyl sulfate, sodium salt sds, is a are potent protein denaturant and solubilizing agent which bind to proteins cancelling differences in intrinsic charge and converting their shape to rodlike particles whose lengths vary uniquely with molecular weights. Sodium lauryl sulphate material safety data sheet cas no. In one article, i found that they used pbs4% formol for fixation step instead of trichloroacetic acidtca, and sodium dodecyl sulfate sds instead of tris base to solubilize protein bound.
Sodium dodecyl sulfate sds, white powder, electrophoresis. Removal of sodium dodecyl sulfate from protein samples. Dodecyl sodium sulfate recommended use laboratory chemicals. Comparison between procedures using sodium dodecyl sulfate. Skin contact wash off immediately with soap and plenty of water removing all contaminated clothes and. Add 100 ml of ethanol 95% and extract the solution with three quantities, each of 50 ml of npentane, adding sodium chloride, if necessary, to promote separation of the two layers. See section 8 of the msds for personal protective equipment. Advice on general occupational hygiene conditions for safe storage, including any incompatibilities eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed.
Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in. Dodecyl sodium sulfate, dodecyl sulfate sodium salt, lauryl sulfate sodium salt, sds, sodium lauryl sulfate. Sodium dodecyl sulfate msds material safety data sheet or sds, coa and coq. Contact can irritate the skin and eyes with possible eye damage.
Effect of light on selfassembly of aqueous mixtures of sodium dodecyl sulfate and a cationic, bolaform surfactant containing azobenzene f. Sdspolyacrylamide gel electrophoresis of purified branchedchain. Request more information about this product request a sample. Sodium dodecyl sulfate sds is an anionic detergent that is used for the solubilization and denaturation of proteins. However, sds interferes with other techniques used for characterization of proteins, such as mass spectrometry ms and amino acid sequencing. Sds is an anionic detergent and wetting agent that is effective in both acid and alkaline. Identification sodium dodecylbenzene sulfonate is a white to light yellow.
Sds solution 10% safety data sheet university of reading. The role of sds in evaporationdriven assembly of nanoparticles have been widely explored 6,29,3742. It is a key component of many reagents used to purify nucleic acids because of its abilities to quickly disrupt the tissue architecture and to inhibit both rnase and deoxyribonuclease dnase activity. First aid measures eye contact rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number. The sds modification on ac was performed at the same time, while cadmium. Show this safety data sheet to the doctor in attendance. It is a component of many cell lysis buffer formulations, including ripa buffer. Western blotting buffers and reagents for protein electrophoresis polyacrylamide gel reagents detergents for protein sample preparation sds sodium dodecyl sulfate. Sodium dodecyl sulfate sds is one of the most popular laboratory reagents used for biological sample extraction. Fine dust dispersed in air in sufficient concentrations, and. See actual entry in rtecs for complete information. This allows for relatively linear separation in electrophoresis, most often page polyacrylamide gel electrophoresis, sodium lauryl sulfate.
Typical sds or lauryl sulfate preparations are mixtures of several different chain lengths c10 to c18. Influence of the sodium dodecyl sulphate sds concentration. Safety data sheet for sodium dodecyl sulfate product number. Identification of the substancemixture and of the companyundertaking 1. The separation of macromolecules in an electric field is called electrophoresis. Breathing sodium dodecylbenzene sulfonate can irritate the nose, throat and lungs causing coughing, wheezing andor shortness of breath. This product is a powder for preparing sds solution. Maccoss department of genome sciences, university of washington, seattle, wa. Specification for sodium lauryl sulfate, nf, ep, bp, jp so180 item number so180 item sodium lauryl sulfate, nf, ep, bp, jp cas number 1512 molecular formula c 12 h 25 o 4 sna molecular weight 288. Causes gastrointestinal irritation with nausea, vomiting and diarrhea. Sodium dodecyl sulfate university of california, davis. Sodium dodecyl sulfate sds, also known as lauryl sulfate, is an ionic detergent that is useful for the rapid disruption of biological membranes. Significant symptoms of exposure can persist for more than two years and can be activated by a variety of. I want to measure the size of the precipitates using.
Sodium dodecyl sulfate cas 1512 817034 merck millipore. Sodium lauryl sulfate can produce allergic sensitivity reactions. Bottle of 20% sodium dodecyl sulfate in distilled water for use in the laboratory. Discharge performance of zincair flow batteries under the. The selected osa starch concentration was 10 % and replacements of a part of the osa starch were realized with sds concentrations of 1, 3 and 5 %. Identification sodium dodecyl sulfate sds, 10% solution sodium dodecyl sulfate sds, 10% solution 3420 central expressway, santa clara ca 95051 3420 central expressway, santa clara ca 95051 trade name. Pdf toxicity of sodium dodecyl sulfate to federally.
Sds is used to denature proteins with a fixed single negative charge per approximately 2 amino acids. Sodium dodecyl sulfate sds is widely used for protein solubilization and for separation of proteins by sds polyacrylamide gel electrophoresis sds. Pdf suppression of sodium dodecyl sulfatepolyacrylamide. Sodium dodecyl sulfate product number l 3771 storage temperature rt product description molecular formula. Product and company identification product name dodecyl sulfate sodium salt cat no. Sodium sulfate, anhydrous 7757 82 6 not listed on the canadian idl ingredient disclosure list 15. Sodium dodecyl sulfate, tested according to nf, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate, 75746 sodium dodecyl sulfate. The anionic surfactant, sodium dodecyl sulfate sds is perhaps one of the most widely studied surfactant with unique physical properties 3336 that has found many applications in science and technology. Other inorganic compounds chemicals fisher scientific. Sample pre treatment solution contains sodium dodecyl sulfate sds in tris buffer 3. Controlled products regulations and the msds contains all of the. Sdspage sodium dodecyl sulfate polyacrylamide gel electrophoresis is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field. Effect of light on selfassembly of aqueous mixtures of. Sci school of civil, environmental and chemical engineering.
Sodium dodecyl sulfate polyacrylamide gel electrophoresis sdspage polyacrylamide gels on the pharmacia phast gel electrophoretic system was performed to investigate the result of inhibitor separations. Popular anionic detergent for a variety of protein methods especially useful for denaturing polyacrylamide gel e. Technical data sheet 299 sodium dodecyl sulfate sds. Sodium dodecyl sulfate an overview sciencedirect topics. Search results for sodium dodecyl sulfate at sigmaaldrich. Weigh accurately about 10 g of sample w and dissolve in 100 ml of water. Dodecyl sodium sulfate dodecyl sulfatesodium salt sds formula. Sodium dodecyl sulfate sds sedimentation is used to determine the relative strength of gluten in a sample of ground wheat or flour. Product and company identification sodium dodecyl sulfate 10% solution synonymsgeneral names. Sodium dodecyl sulfate definition of sodium dodecyl sulfate. Influence of the sodium dodecyl sulphate sds concentration on. Discharge performance of zincair flow batteries under the effects of sodium dodecyl sulfate and pluronic f127 skip to main content thank you for visiting.
A chemical safety assessmentreport csacsr has not been. Application of sodium dodecyl sulfateactivated carbon. Sodium dodecyl sulfate sds safety data sheet for sodium dodecyl sulfate 817034 material safety data sheet or sds for sodium dodecyl sulfate 817034 from merck for download or viewing in the browser. Only select registry of toxic effects of chemical substances rtecs data is presented here. The information given is designed only as a guide for safe handling, use, processing, storage. Sodium dodecylbenzenesulfonate 25155300 40 sodium sulfate 7757826 40 sodium chloride 7647145 10 dodecyl benzene sulphonic acid sodium salt cas no 25155300 material safety data sheet sds msds.
It is also incompatible with cationic materials and with acids with ph below 2. A formulation containing 15% ammonium lauryl sulfate caused depression, labored breathing, diarrhea, and death in four out of 20 animals. Sodium dodecyl sulfate sds or sodium lauryl sulfate sls, sometimes written sodium laurilsulfate, is a synthetic organic compound with the formula c h 3 ch 2 11 so 4 na. Sdspage is an electrophoresis method that allows protein separation by mass. Sigmaaldrich offers a number of sodium dodecyl sulfate products. Sodium lauryl sulfate increases the per meability of the skin. This sds does not contain any contaminating ions that might impair proper electrophoretic mobility of test samples. The effect of sodium dodecyl sulphate sds and sodium dodecylbenzene sulphonate sdbs on activated sludge oxygen uptake rate our and nitrification a thesis submitted in fulfilment of the requirements for the degree of master of engineering yi jiao b. Sds of sodium dodecyl sulfate, safety data sheets, cas 1512. Sa mple diluent contains 5% bovine serum albumin in phosphate buffer with 0. Ntp, 1992 salts, basic, such as dodecyl sulfate, are generally soluble in water.
Sodium dodecyl sulphate substance information echa. The oil contents of the examined emulsions were 5, 20 and 50 %. Sodium dodecylbenzene sulfonate can affect you when breathed in. Sds of sodium dodecyl sulfate, safety data sheets, cas 151.
Sodium lauryl sulfate 1512 85 sodium sulfate 7757826 6. Sodium dodecyl sulfate sds is an anionic detergent used to solubilize and denature proteins in protein extraction and sdspage. Abbott department of chemical and biological engineering, university of madison wisconsin, 1415 engineering drive, madison, wisconsin 537061691. Sodium dodecyl sulphate is catagorised as wgk 2 under the german water classification regulations vwvws. Susceptible to 1% sodium hypochlorite, 2% glutaraldehyde, 0. Product name and description sodium dodecyl sulphate sulfate solution 10% trade namebrand not applicable synonyms sds solution. Sodium dodecyl sulfate classified as a flammable solid according to dot test methods. Sodium dodecyl sulfate is used in general as a detergent, dispersant, and surfactant. Sodium dodecyl sulfate sds is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. Sodium dodecyl sulfate microaggregates with diversely. Thermo scientific sodium dodecyl sulfate lauryl is standardgrade sds detergent for use in protein polyacrylamide gel electrophoresis page.
682 1370 669 1300 230 937 1343 210 626 1296 569 828 540 403 1506 707 453 1329 1491 1181 523 1110 540 783 881 571 1243 717 841 657 343